Journal: Frontiers in Bioengineering and Biotechnology
Article Title: The hybrid crosslinking method improved the stability and anti-calcification properties of the bioprosthetic heart valves
doi: 10.3389/fbioe.2022.1008664
Figure Lengend Snippet: Characterization of the host response of various samples after subcutaneous implantation for 1 week and 3 weeks. The images show the immunohistochemical staining of CD68 and CD3 (A,B,C,D) , on the right side of the corresponding image is the semi-quantitative result (E–H) . In the first week, CD3 + cells were not significantly different between the GA and NPTD groups, while in the third week, there were fewer CD3 positive cells in the NPTD group than in the GA group. The results showed the reduced CD68 + inflammatory cell infiltration in the NPTD group compared to the GA and NA groups at 1 week and 3 weeks. Statistical analysis was performed by one-way analysis of variance (ANOVA).
Article Snippet: Rabbit anti-rat CD68 antibody (dilution 1:400; Servicebio, GB11067) was used to label the macrophage cells, and rabbit anti-rat CD3 antibody (dilution 1:700; Servicebio, GB11014) was used to label the T cells.
Techniques: Immunohistochemical staining, Staining